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I can't really say how long the heart keeps beating or how long the circulatory system stays pressurized as it is not my area of expertise. However, I can say the 8-10 animals I have stuck whether immediately or 10 minutes after shot, gush blood out their new orifice and show markedly less blood in the gut cavity. Before I stick them I also try and orient them head downhill to let gravity help and maybe it works somewhat like a siphon effect? Try it and see if it works for yourself!